Application Note: Design of a multicolumn process for mAb capture

Mechanistic simulation to assess the impact of the number of columns on key performance indicators

• by Ypso-Facto
• October 13, 2022

There is an increasing interest in using multicolumn processes for the capture of monoclonal antibobies (mAbs) by affinity chromatography. However, selecting and designing the most suitable process is not straightforward. There are indeed a wide range of process configurations, with processes ranging from 1 up to 4 or 5 columns.

In this case study, we use mechanistic simulation as a tool to rationally compare single-column and multicolumn processes for mAb capture. We assess the impact of the number of columns on key performance indicators like recovery and productivity.

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